Journal: Journal of Cellular and Molecular Medicine

Article Title: Single‐cell RNA‐seq reveals altered NK cell subsets and reduced levels of cytotoxic molecules in patients with ankylosing spondylitis

doi: 10.1111/jcmm.17159

Figure Lengend Snippet: Expression of cytolytic molecules in plasma of HCs ( n = 16) and AS patients ( n = 16). The plasma levels of granzyme A (A), granzyme B (B) and granulysin (C) were determined by enzyme‐linked immunosorbent assay. Pearson correlation analysis was performed between granzyme A (D), granzyme B (E), granulysin (F) and disease activity (BASDAI). Horizontal lines and error bars show the mean ± SEM. ** p < 0.01; *** p < 0.001; ns = not significant

Article Snippet: Plasma levels of cytotoxic granules, including GZMA, GZMB and granulysin, were quantified using ELISA kits (#EK1162, #EK1114 and # EK1280, BOSTER Biological Technology).

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Activity Assay

Journal: BMC Medical Genetics

Article Title: First mutation in the FSHR cytoplasmic tail identified in a non-pregnant woman with spontaneous ovarian hyperstimulation syndrome

doi: 10.1186/s12881-017-0407-6

Figure Lengend Snippet: Functional studies of the wild-type and mutant FSHR. COS-7 cells were transfected with empty (mock) or expression vector encoding WT and/or mutated receptor. COS-7 cells were transfected with empty (mock) or expression vector encoding WT and/or mutated receptor. a Cyclic AMP (cAMP) level observed in transfected cells in the absence of any hormonal stimulation. Graph represents the results of at least three separate experiments performed in duplicate conditions. b and c Transfected cells were incubated with increasing concentrations of human follicle-stimulating hormone (FSH) and the accumulation of cAMP was measured. Each graph represents the results (mean ± SEM) of at least two separate experiments. d Cell surface expression of WT and mutated FSHRs. Permeabilized (P) or nonpermeabilized (NP) transfected cells were incubated with the FSHR323 antibody. Confocal microscopy was used to study the cellular distribution of receptors. e The membrane expression of the FSHR R634H is decreased. COS-7 cells were transfected with WT or mutated FSHR. Immunoprecipitation of FSHR from total extract (total) or from cell surface (surface) was performed using FSHR323. Immunoprecipitated FSHR was analyzed by western-blotting. Size of the molecular mass is indicated

Article Snippet: Forty-eight hours after transfection, the intracellular accumulation of cyclic AMP (cAMP) was measured after incubation for 45 min with various concentrations of recombinant human FSH (Gonal-F®, Merck, France) using cAMP complete ELISA kit (Enzo Life Sciences).

Techniques: Functional Assay, Mutagenesis, Transfection, Expressing, Plasmid Preparation, Incubation, Confocal Microscopy, Immunoprecipitation, Western Blot